Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea
نویسندگان
چکیده
منابع مشابه
Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea
This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler's diarrhea and are resistant to standard antimicrobial treatments. The target genes included the Cryptosporidium oocyst wall protein for C. parvum, Glutamate dehydrogenase ...
متن کاملDevelopment of a Diagnostic Kit to Detect Cryptosporidium parvum and Giardia lamblia
OBJECTIVES This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of Cryptospofidium parvum and Giardia lamblia in patients with diarrhea. METHODS The cultivated C. parvum oocysts and G. lamblia cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were sepa...
متن کاملCryptosporidium parvum and Cyclospora cayetanensis: a review of laboratory methods for detection of these waterborne parasites.
Cryptosporidium and Cyclospora are obligate, intracellular, coccidian protozoan parasites that infest the gastrointestinal tract of humans and animals causing severe diarrhea illness. In this paper, we present an overview of the conventional and more novel techniques that are currently available to detect Cryptosporidium and Cyclospora in water. Conventional techniques and new immunological and...
متن کاملPrevalence of infection with Cryptosporidium parvum and Cyclospora cayetanensis among international travellers.
BACKGROUND Cryptosporidium parvum and Cyclospora cayetanensis are recognised as possible pathogens of traveller's diarrhoea. AIMS To identify the prevalence of C parvum and Cyc cayetanensis in travellers returning from developing countries. PATIENTS Nine hundred and seventy eight stool samples wer taken from 795 patients returning from developing countries. METHODS Microscopy (iron-haemat...
متن کاملDirect Molecular Detection and Phylogenetic Tree Analysis of Gastrointestinal Protozoan Parasites (Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum) from Diarrhea Infection in Kut City of Iraq: A Short Communication
Background: The intestinal tract of human can be infected by protozoan parasites. In this short communication, the stool samples were collected from patients with diarrhea referred to Kut hospital, Iraq, and then the parasites (Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum) were considered for molecular identification. Methods: Stool samples were collected from 69 patients wit...
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ژورنال
عنوان ژورنال: The Korean Journal of Parasitology
سال: 2016
ISSN: 0023-4001,1738-0006
DOI: 10.3347/kjp.2016.54.5.631